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. 2011 May;22(1):91-8.

A Comparison between Hippurate Hydrolysis and Multiplex PCR for Differentiating Campylobacter coli and Campylobacter jejuni

Frederick Adzitey  1 , Janet Corry  2
Affiliations

A Comparison between Hippurate Hydrolysis and Multiplex PCR for Differentiating Campylobacter coli and Campylobacter jejuni

Frederick Adzitey et al. Trop Life Sci Res. 2011 May.

Abstract
in English, Indonesian

Species identification is important for epidemiological, clinical and treatment purposes. The aim of this study was to find out whether hippurate hydrolysis is a reliable test for differentiating between Campylobacter coli and Campylobacter jejuni. To achieve this, hippurate hydrolysis test was compared with multiplex Polymerase Chain Reaction (mPCR) for their ability to speciate C. coli and C. jejuni. Eighteen Campylobacter strains from poultry samples were used for this study. The results from 17 of the 18 strains were in agreement with both methods. Thus, the hippurate hydrolysis test can be used for distinguishing C. jejuni from C. coli although occasionally some strains of C. jejuni may be mis-identified as C. coli.

Pengenalpastian spesies penting untuk tujuan epidemiologikal, klinikal dan perubatan. Tujuan kajian ini adalah untuk mengetahui sama ada ujian hidrolisis hippurate berkesan untuk membezakan antara Campylobacter coli dan Campylobacter jejuni. Untuk mencapai ini, ujian hidrolis hippurate dibandingkan dengan multiplex Polymerase Chain Reaction (mPCR) untuk mengkaji kebolehan membezakan spesies C. coli dan C. jejuni. Lapan belas strain Campylobacter daripada sampel haiwan ternakan digunakan. Keputusan 17 daripada 18 strain menunjukkan keberkesanan menggunakan kedua-dua jenis ujian. Maka ujian hidrolisis hippurate berkesan untuk membezakan C. jejuni daripada C. coli, walaupun kadang-kadang terdapat strain C. jejuni yang diidentifikasi secara salah sebagai C. coli.

Keywords: C. coli; C. jejuni; Hippurate Hydrolysis; mPCR.

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Figures

Figure 1:
Figure 1:
Representative results of hippurate hydrolysis test carried out on microtitre plate. Note: Deep purple colour indicates C. jejuni while medium or no colour change indicates C. coli.
Figure 2:
Figure 2:
A representative mPCR assay showing results for 18 Campylobacter isolates from poultry-related samples. Lane 1, C. coli; lanes 2–4, C. jejuni; lanes 5–14, C. coli; lanes 15–18, C. jejuni (lane 17, has a missing hipO band); lane 19, C. jejuni (11168) positive control; lane 20, C. coli (RM28) negative control; lane 21, negative control. Notes: Lanes 1–2, were medium purple (C. coli); lanes 3–4, were deep purple (C. jejuni); lanes 5–14, were medium purple (C. coli); 15–18, were deep purple (C. jejuni) by hippurate hydrolysis test.
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