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. 2014 May;52(4):433-7.
doi: 10.1093/mmy/myt017. Epub 2014 Jan 3.

Usefulness of pan-fungal NASBA test for surveillance of environmental fungal contamination in a protected hematology unit

Marie-Pierre Brenier-Pinchart  1 , Hafid Abaibou, Thomas Berendsen, Gautier Szymanski, Messalia Beghri, Sébatien Bailly, Frédérick Lasnet, Anne Thiebaut-Bertrand, Claude Mabilat, Hervé Pelloux
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Usefulness of pan-fungal NASBA test for surveillance of environmental fungal contamination in a protected hematology unit

Marie-Pierre Brenier-Pinchart et al. Med Mycol. 2014 May.

Abstract

A pan-fungal nucleic acid sequence based applification (NASBA) test was adapted and used for the first time to detect and quantify the level of filamentous fungi in environmental samples. Surface samples (n = 356) collected in a controlled air flow hematology ward were tested by mycological culture and the pan-fungal NASBA test. The overall percentage of agreement between culture and NASBA was 88%, the Kappa coefficient was equal to 0.61 (95%CI = [0.51; 0.72]). This pan-fungal NASBA test could be a promising tool to rapidly monitor the absence of molds in controlled environments.

Keywords: NASBA; hematology unit; invasive aspergillosis; molds; protected environments.

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