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. 2014 Apr 11;50(28):3747-9.
doi: 10.1039/c4cc00540f. Epub 2014 Feb 28.

Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)

Kuangwen Hsieh  1 , Peter L Mage, Andrew T Csordas, Michael Eisenstein, H Tom Soh
Affiliations

Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP)

Kuangwen Hsieh et al. Chem Commun (Camb). .

Abstract

We report a one-pot, closed-vessel enzymatic assay that eliminates carryover contamination while preserving robust DNA amplification in loop-mediated isothermal amplification (LAMP), providing reliable and rapid detection of target DNA in contaminated samples.

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