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. 2014 Jun;25(6):1577-87.
doi: 10.1007/s10856-014-5179-3. Epub 2014 Feb 28.

The effects of micro arc oxidation of gamma titanium aluminide surfaces on osteoblast adhesion and differentiation

Pricilla Santiago-Medina  1 , Paul A Sundaram, Nanette Diffoot-Carlo
Affiliations

The effects of micro arc oxidation of gamma titanium aluminide surfaces on osteoblast adhesion and differentiation

Pricilla Santiago-Medina et al. J Mater Sci Mater Med. 2014 Jun.

Abstract

The adhesion and proliferation of human fetal osteoblasts, hFOB 1.19, on micro arc oxidized (MAO) gamma titanium aluminide (γTiAl) surfaces were examined in vitro. Cells were seeded on MAO treated γTiAl disks and incubated for 3 days at 33.5 °C and subsequently for 7 days at 39.5 °C. Samples were then analyzed by scanning electron microscopy (SEM) and alkaline phosphatase assay (ALP) to evaluate cell adhesion and differentiation, respectively. Similar Ti-6Al-4V alloy samples were used for comparison. Untreated γTiAl and Ti-6Al-4V disks to study the effect of micro arc oxidation and glass coverslips as cell growth controls were also incubated concurrently. The ALP Assay results, at 10 days post seeding, showed significant differences in cell differentiation, with P values <0.05 between MAO γTiAl and MAO Ti-6Al-4V with respect to the corresponding untreated alloys. While SEM images showed that hFOB 1.19 cells adhered and proliferated on all MAO and untreated surfaces, as well as on glass coverslips at 10 days post seeding, cell differentiation, determined by the ALP assay, was significantly higher for the MAO alloys.

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Figures

Fig 1
Fig 1
SEM images of the Micro Arc Oxidation surfaces for different process conditions: (a) γTiAl, 200 mA, 3 min, (b) Ti-6Al-4V, 200 mA, 3 min, (c) γTiAl, 200 mA, 4 min, (d) Ti-6Al-4V, 200 mA, 4 min, (e) γTiAl, 225 mA, 3 min, (f) Ti-6Al-4V, 225 mA, 3 min, (g) γTiAl, 225 mA, 4 min, (b) Ti-6Al-4V, 225 mA, 4 min
Fig 1
Fig 1
SEM images of the Micro Arc Oxidation surfaces for different process conditions: (a) γTiAl, 200 mA, 3 min, (b) Ti-6Al-4V, 200 mA, 3 min, (c) γTiAl, 200 mA, 4 min, (d) Ti-6Al-4V, 200 mA, 4 min, (e) γTiAl, 225 mA, 3 min, (f) Ti-6Al-4V, 225 mA, 3 min, (g) γTiAl, 225 mA, 4 min, (b) Ti-6Al-4V, 225 mA, 4 min
Fig 2
Fig 2
SEM images of hFOB1.19 cell attachment on MAO surfaces for different process conditions: (a) γTiAl, 200 mA, 3 min, (b) Ti-6Al-4V, 200 mA, 3 min, (c) γTiAl, 200 mA, 4 min, (d) Ti-6Al-4V, 200 mA, 4 min, (e) γTiAl, 225 mA, 3 min, (f) Ti-6Al-4V, 225 mA, 3 min, (g) γTiAl, 225 mA, 4 min, (b) Ti-6Al-4V, 225 mA, 4 min
Fig 3
Fig 3
Morphological characterization of micro oxidized γTiAl and Ti-6Al-4V alloys by Atomic Force Microscopy. (a) γTiAl, 200 mA, 3 min, (b) Ti-6Al-4V, 200 mA, 3 min, (c) γTiAl, 200 mA, 4 min, (d) Ti-6Al-4V, 200 mA, 4 min, (e) γTiAl, 225 mA, 3 min, (f) Ti-6Al-4V, 225 mA, 3 min, (g) γTiAl, 225 mA, 4 min, (b) Ti-6Al-4V, 225 mA, 4 min
Fig 3
Fig 3
Morphological characterization of micro oxidized γTiAl and Ti-6Al-4V alloys by Atomic Force Microscopy. (a) γTiAl, 200 mA, 3 min, (b) Ti-6Al-4V, 200 mA, 3 min, (c) γTiAl, 200 mA, 4 min, (d) Ti-6Al-4V, 200 mA, 4 min, (e) γTiAl, 225 mA, 3 min, (f) Ti-6Al-4V, 225 mA, 3 min, (g) γTiAl, 225 mA, 4 min, (b) Ti-6Al-4V, 225 mA, 4 min
Fig 4
Fig 4
pNPP Standard curve used to extrapolate pNPP concentration to determine ALP activity
Fig 5
Fig 5
Alkaline phosphatase activity (n=12) on micro arc oxidized γTiAl and Ti-6Al-4V alloys for different treatment conditions. Note ALP data for glass coverslips and untreated γTiAl and Ti-6Al-4V are plotted for comparison
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