Developmental expression of platelet-derived growth factor and its receptor in the human placenta

Abstract

To investigate the role of platelet-derived growth factor (PDGF) during human placental development, expression of the genes encoding PDGF, the PDGF-receptor (PDGF-R) and the c-fos protooncogene was measured. Messenger RNAs for these genes were detected throughout pregnancy and peaked coordinately during the second trimester. An identical pattern of PDGF-R protein expression was confirmed by immunoblotting using a specific PDGF-R antiserum, measurement of PDGF-R kinase activity, and [125I]PDGF binding. These findings show that the components of the PDGF pathway are expressed in a concerted fashion throughout human pregnancy and are present at especially high levels during the midtrimester. Our observations suggest that through autocrine and/or paracrine mechanisms, PDGF is likely to play an important role in placental homeostasis.