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. 2011;64(4):252-5.
doi: 10.5173/ceju.2011.04.art14. Epub 2011 Dec 9.

Immunohistochemical detection of angiotensin AT 1 and AT 2 receptors in prostate cancer

Marek Pawlikowski  1 , Radosław Minias  2 , Marek Sosnowski  3 , Krzysztof W Zieliński  2
Affiliations

Immunohistochemical detection of angiotensin AT 1 and AT 2 receptors in prostate cancer

Marek Pawlikowski et al. Cent European J Urol. 2011.

Abstract

Introduction: The human prostate gland contains all the compounds of the renin-angiotensin system (RAS), including AT1 and AT2 angiotensin receptors. The role of local RAS in the prostate pathology is recently discussed. The aim of the present study was the evaluation of AT1 and AT2 expressions in human prostate cancers.

Material and methods: The investigation was performed in 20 paraffin-embedded needle biopsy specimens from routine diagnostic prostate cancer biopsies. The specimens were stained with hematoxylin and eosin and immunostained with anti-AT1 and anti-AT2 antibodies. For visualization of primary antibodies, the streptavidin-biotin-peroxidase technique was applied. The expression of both receptor proteins was evaluated quantitatively using image analysis method.

Results: The positive immunostaining with both anti- AT1 and anti-AT2 antibodies can be found in stromal as well as epithelial structures. The results of quantitative evaluation showed the positive correlation between AT1 and AT2 expressions in neoplastic epithelium and overexpression of both AT1 and AT2 in neoplastic epithelium of Gleason grade 2, but not in cancerous structures of Gleason grades 3-5.

Conclusions: The data on AT1 and AT2 receptor expressions may suggest the involvement of RAS in prostate cancerogenesis. Moreover, the persistence of AT1 receptors in prostate cancer speaks in favor of attempts to use of AT1 receptor blockers (i.e. sartanes) and/or AT2 agonists in prostate cancer prophylaxis and/or treatment.

Keywords: AT1; AT2; angiotensin receptors; immunohistochemistry; prostate cancer; quantitative evaluation.

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Figures

Fig. 1
Fig. 1
An assessment image of a Gleason 3 carcinomatous infiltration (H&E, magn. 50x).
Fig. 2
Fig. 2
The compatible image with AT1 immunohistochemical reaction in carcinomatous infiltration (DAB, 100x; insert: the magnified carcinomatous tubule, 400x).
Fig. 3
Fig. 3
The resulting image for densitometric measurement of DAB expressions in the carcinomatous tubule from Fig. 2.
Fig. 4
Fig. 4
The densitometric ratio of DAB reaction intensity of red cell and normal or neoplastic epithelium.
See this image and copyright information in PMC

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References

    1. O'Mahony OA, Barker S, Puddefoot JR, Vinson GP. Synthesis and secretion of angiotensin II by the prostate gland in vitro. Endocrinology. 2005;146:392–398. - PubMed
    1. Dinh DT, Frauman AG, Sourial M, et al. Identification, distribution, and expression of angiotensin II receptors in the normal human prostate and benign prostatic hyperplasia. Endocrinology. 2001;142:1349–1356. - PubMed
    1. Pawlikowski M, Melen-Mucha G, Mucha S. The involvement of angiotensins in the control of prostatic epithelial cell proliferation in the rat. Folia Histochem Cytobiol. 2001;39:341–343. - PubMed
    1. Nassis I, Frauman AG, Ohishi M, et al. Localization of angiotensin-converting enzyme in human prostate: pathological expression in benign prostatic hyperplasia. J Pathol. 2001;195:571–579. - PubMed
    1. Dinh DT, Frauman AG, Somers GR, et al. Evidence for activation of the reninangiotensin system in the human prostate: increased angiotensin II and reduced AT(1) receptor expression in benign prostatic hyperplasia. J Pathol. 2002;196:213–219. - PubMed

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