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. 1988 Jun 1;252(2):545-51.
doi: 10.1042/bj2520545.

Solubilization and characterization of substance P-binding sites from chick brain membranes

H P Too  1 , M R Hanley
Affiliations

Solubilization and characterization of substance P-binding sites from chick brain membranes

H P Too et al. Biochem J. .

Abstract

Sites binding monoiodinated-Bolton-Hunter-reagent-labelled substance P were solubilized from 1-day-old-chick brain membrane by using non-ionic detergents (1% digitonin/1% n-octyl glucoside) and a high concentration of NaCl (0.5 M). The solubilized preparation retained the pharmacological properties of the high-affinity binding sites found in the native membrane. The high density of specific binding sites (approximately 2 pmol of binding sites/mg of protein) suggests that the chick brain membranes may be a useful source for the purification of the substance P-binding sites.

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References

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