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. 1988 Jan:89 ( Pt 1):21-4.
doi: 10.1242/jcs.89.1.21.

Coumarin-phalloidin: a new actin probe permitting triple immunofluorescence microscopy of the cytoskeleton

J V Small  1 , S Zobeley, G Rinnerthaler, H Faulstich
Affiliations

Coumarin-phalloidin: a new actin probe permitting triple immunofluorescence microscopy of the cytoskeleton

J V Small et al. J Cell Sci. 1988 Jan.

Abstract

7-Diethylamino-3-(4-isothiocyanotophenyl)-4-methylcoumarin (CPITC) was coupled to amino-methyldithiolanophalloidin to produce a new phalloidin derivative, coumarin-phalloidin, fluorescent in the blue region of the spectrum. Coumarin-phalloidin binds to actin with around 100-fold less affinity than unconjugated phalloidin, but with enough avidity to make it a useful stain for actin filaments. Appropriate filter combinations permit triple immunofluorescence microscopy of the cytoskeleton with fluorescein and rhodamine conjugates together with coumarin-phalloidin.

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