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. 2014 Apr;11(4):399-402.
doi: 10.1038/nmeth.2857. Epub 2014 Mar 2.

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects

Bin Shen  1 , Wensheng Zhang  2 , Jun Zhang  1 , Jiankui Zhou  3 , Jianying Wang  3 , Li Chen  3 , Lu Wang  4 , Alex Hodgkins  5 , Vivek Iyer  5 , Xingxu Huang  3 , William C Skarnes  5
Affiliations

Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects

Bin Shen et al. Nat Methods. 2014 Apr.

Abstract

Bacterial RNA-directed Cas9 endonuclease is a versatile tool for site-specific genome modification in eukaryotes. Co-microinjection of mouse embryos with Cas9 mRNA and single guide RNAs induces on-target and off-target mutations that are transmissible to offspring. However, Cas9 nickase can be used to efficiently mutate genes without detectable damage at known off-target sites. This method is applicable for genome editing of any model organism and minimizes confounding problems of off-target mutations.

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References

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