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. 2014:1125:313-24.
doi: 10.1007/978-1-62703-971-0_25.

Assaying mRNA deadenylation in vivo

Claudia Temme  1 , Elmar Wahle
Affiliations

Assaying mRNA deadenylation in vivo

Claudia Temme et al. Methods Mol Biol. 2014.

Abstract

Deadenylation is the removal of poly(A) tails from mRNA. Here, we present two methods for assaying deadenylation in vivo. The first is a method for measuring bulk poly(A) tail lengths. When combined with a block in transcription, the method can be used for measuring the rate of bulk poly(A) tail shortening. The second is an RT-PCR method to determine the poly(A) tail lengths of individual RNAs. Again in combination with a block of transcription, the method permits the rate of deadenylation of an individual RNA to be measured.

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