Electrophoretic heterogeneity of lipopolysaccharides of Actinobacillus actinomycetemcomitans

Abstract

Electrophoretic banding patterns of lipopolysaccharides (LPS), as observed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), have proved to be useful in studies relating LPS structure to virulence and as epidemiological markers. In this report, LPS of actinobacillus actinomycetemcomitans from outer membrane fractions and hot phenol-water extracts were analyzed by SDS-PAGE and LPS-specific silver-staining techniques. Both intra- and inter-strain heterogeneity of A. actinomycetemcomitans LPS was observed. Twelve strains of A. actinomycetemcomitans, representative of the three described serotypes, were assigned to LPS subtypes based on the relative mobility of their most rapidly migrating LPS band. All three serotype a strains (29523, aB75, and GA3), two (29524 and SAC11A) of five serotype b strains, and two (aB67 and SAC5A) of four serotype c strains were assigned to LPS subtype I. The three remaining serotype b strains (29522, Y4, and JP2) were assigned to LPS subtype II, and the remaining two serotype c strains (SAC6A and SAC12A) were assigned to LPS subtype III. LPS subtyping may serve as an adjunct or alternative to serotyping in epidemiological studies.