Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Nov;62(11):4410-3.
doi: 10.1128/JVI.62.11.4410-4413.1988.

Transactivating potential of the 3' open reading frame of murine mammary tumor virus

P van Klaveren  1 , P Bentvelzen
Affiliations

Transactivating potential of the 3' open reading frame of murine mammary tumor virus

P van Klaveren et al. J Virol. 1988 Nov.

Abstract

The procaryotic chloramphenicol acetyltransferase (CAT) gene controlled by the murine mammary tumor virus (MMTV) promoter shows reduced activity in a rat mammary tumor cell line after infection with MMTV but to a considerably lesser extent than the CAT gene controlled by a heterologous promoter, indicating trans-acting regulation of promoter activity by MMTV. Cotransfection of vectors capable of expressing RNA from the 3' open reading frame (orf) of MMTV with the CAT-MMTV construct resulted in enhanced CAT activity, suggesting that orf carries a transactivating potential. Since transactivation was also found with a vector containing only orf and part of the viral env gene, it was concluded that a separate transcriptional unit exists for the orf message.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1978 Nov;75(11):5718-22 - PubMed
    1. J Virol. 1988 Apr;62(4):1373-80 - PubMed
    1. J Virol. 1981 Jan;37(1):226-38 - PubMed
    1. J Virol. 1981 Jan;37(1):36-47 - PubMed
    1. Nature. 1981 Jun 11;291(5815):511-3 - PubMed

LinkOut - more resources