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. 2013;17(3):250-6.
doi: 10.5114/wo.2013.35278. Epub 2013 Jun 28.

Expression of β-catenin and AXIN2 in ameloblastomas

Zhenhui Wei  1 , Ming Zhong  2 , Yan Guo  3 , Yan Wang  4 , Meisi Ren  2 , Zhaoyuan Wang  2
Affiliations

Expression of β-catenin and AXIN2 in ameloblastomas

Zhenhui Wei et al. Contemp Oncol (Pozn). 2013.

Abstract

Aim of the study: To investigate the expression status and association of β-catenin and AXIN2 in ameloblastoma.

Material and methods: 30 ameloblastoma specimens and 10 normal oral mucosa tissues were enrolled in the study. The protein and RNA levels of β-catenin and AXIN2 were detected by immunohistochemistry staining, Western blot, and real-time PCR analysis. The relationship between β-catenin and AXIN2 protein and clinico-pathological parameters and prognosis was subsequently determined.

Results: The results show that β-catenin mRNA expression was 1.24-fold higher in ameloblastomas than that in normal mucosa tissues, but AXIN2 mRNA expression was 0.47-fold lower in ameloblastomas than that in normal mucosa tissues (p < 0.05). The Western blot results show that β-catenin and AXIN2 protein in ameloblastomas had a significantly higher level than normal mucosa tissues (p < 0.05). Immunohistochemical staining of β-catenin and AXIN2 protein in ameloblastoma was expressed at a higher level than normal oral mucosa (p < 0.05).

Conclusions: AXIN2 and β-catenin play an important role in the tumorigenesis and progression of ameloblastoma.

Keywords: AXIN2; Wnt pathway; ameloblastoma; β-catenin.

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Figures

Fig. 1
Fig. 1
Amplification and dissociation curves detected by real-time PCR; A) CTNNB1 amplification; B) CTNNB1 dissociation; C) AXIN2 amplification; D) AXIN2 dissociation curve
Fig. 2
Fig. 2
A) β-catenin expression level detected by Western blot analysis. The figures below the gel image represent the ratios of β-catenin to β-tubulin; B) AXIN2 expression level detected by Western blot analysis. The figures below the gel images represent the ratios of AXIN2 to β-tubulin
Fig. 3
Fig. 3
A) β-catenin aberrant expression in cytoplasm of ameloblastomas (20×); B) β-catenin aberrant expression in the nucleus of ameloblastomas (arrow) (40×); C) β-catenin expressed in the normal oral mucous membrane, with no aberrant expression in cytoplasm and nucleus (20×); D) AXIN2 is expressed in ameloblastomas (20×); E) AXIN2 is expressed in ameloblastomas (40×); F) AXIN2 is expressed in the normal oral mucous membrane (20×)
See this image and copyright information in PMC

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