Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Sep;7(9):2697-703.
doi: 10.1002/j.1460-2075.1988.tb03123.x.

The structure of cytochrome b561, a secretory vesicle-specific electron transport protein

Affiliations

The structure of cytochrome b561, a secretory vesicle-specific electron transport protein

M S Perin et al. EMBO J. 1988 Sep.

Abstract

Cytochrome b561 is a transmembrane electron transport protein that is specific to a subset of secretory vesicles containing catecholamines and amidated peptides. This protein is thought to supply reducing equivalents to the intravesicular enzymes dopamine-beta-hydroxylase and alpha-peptide amidase. We have purified cytochrome b561 from bovine adrenal chromaffin granules by reverse phase chromatography and have determined internal amino acid sequences from peptides. Complementary oligonucleotides were used to isolate two cDNA clones from a bovine brain library. The structure predicted by the sequences of these cDNAs suggests a highly hydrophobic protein of 273 amino acids which spans the membrane six times with little extramembranous sequence. Cytochrome b561 is not homologous to any other cytochrome and thus represents a new class of electron carriers. RNA blotting experiments indicate that cytochrome b561 is expressed in the adrenal medulla and all brain regions of the cow, but not in visceral organs. This result agrees well with the putative function of this unique cytochrome and with the notion that this protein is localized to large dense-core synaptic vesicles.

PubMed Disclaimer

References

    1. J Biol Chem. 1983 Jan 10;258(1):27-30 - PubMed
    1. Biochem J. 1967 May;103(2):480-2 - PubMed
    1. J Biol Chem. 1986 Jul 25;261(21):9746-52 - PubMed
    1. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 - PubMed
    1. EMBO J. 1986 Nov;5(11):3021-7 - PubMed

Publication types

LinkOut - more resources