Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 1988 Sep;26(9):1735-7.
doi: 10.1128/jcm.26.9.1735-1737.1988.

Staining characteristics of six commercially available monoclonal immunofluorescence reagents for direct diagnosis of Chlamydia trachomatis infections

L D Cles  1 , K Bruch, W E Stamm
Affiliations
Comparative Study

Staining characteristics of six commercially available monoclonal immunofluorescence reagents for direct diagnosis of Chlamydia trachomatis infections

L D Cles et al. J Clin Microbiol. 1988 Sep.

Abstract

Using purified elementary bodies of 14 Chlamydia trachomatis serovars in an in vitro assay, we compared the staining characteristics of six commercially available monoclonal antibody reagents used for direct immunofluorescent staining of patient smears. Considerable variation in the degree of brightness, consistency of staining, and specificity of the six reagents was found. Monoclonal antibodies against the major outer membrane proteins of C. trachomatis produced brighter fluorescence, more consistent elementary body morphology, and less nonspecific staining than did monoclonal antibodies directed against chlamydial lipopolysaccharide.

PubMed Disclaimer

References

    1. J Clin Microbiol. 1979 Aug;10(2):259-61 - PubMed
    1. J Immunol. 1982 Mar;128(3):1083-9 - PubMed
    1. N Engl J Med. 1984 May 3;310(18):1146-50 - PubMed
    1. Ann Intern Med. 1984 Nov;101(5):638-41 - PubMed
    1. J Clin Microbiol. 1984 Nov;20(5):948-50 - PubMed

Publication types

MeSH terms