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Comparative Study
. 1988 Dec;62(12):4786-90.
doi: 10.1128/JVI.62.12.4786-4790.1988.

Hepatitis B virus particles contain a polypeptide encoded by the largest open reading frame: a putative reverse transcriptase

D H Mack  1 , W Bloch, N Nath, J J Sninsky
Affiliations
Comparative Study

Hepatitis B virus particles contain a polypeptide encoded by the largest open reading frame: a putative reverse transcriptase

D H Mack et al. J Virol. 1988 Dec.

Abstract

A segment of the largest open reading frame of hepatitis B virus (HBV) was inserted into an open reading frame vector directing the expression in Escherichia coli of a fusion molecule containing 143 HBV-encoded amino acids. The fusion protein was used to generate antiserum which served in immunoblots to identify a polypeptide with a molecular mass of 65 kilodaltons in HBV particles. Because of the small number of molecules in virus particles, unambiguous detection required the development of a highly sensitive immunoblot procedure.

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References

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