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. 2014 Mar:452-453:237-46.
doi: 10.1016/j.virol.2014.01.015. Epub 2014 Feb 14.

Viral kinetics of primary dengue virus infection in non-human primates: a systematic review and individual pooled analysis

Benjamin M Althouse  1 , Anna P Durbin  2 , Kathryn A Hanley  3 , Scott B Halstead  4 , Scott C Weaver  5 , Derek A T Cummings  2
Affiliations

Viral kinetics of primary dengue virus infection in non-human primates: a systematic review and individual pooled analysis

Benjamin M Althouse et al. Virology. 2014 Mar.

Abstract

Viremia kinetics directly influence the clinical course and transmission dynamics of DENV, but many aspects of viral dynamics remain unknown. Non-human primates (NHP) have been used as a model system for DENV infection for decades. Here, we identify papers with experimentally-infected NHP and estimate the time to- and duration of viremia as well as estimate associations between these and serotype, inoculating dose, viremia assay, and species of NHP. We estimate the time to viremia in rhesus macaques to range from 2.63 to 3.32 days for DENV-2 and -1 and the duration to range from 3.13 to 5.13 days for DENV-4 and -2. We find no differences between non-human primates for time to viremia or duration, and a significant negative relationship between inoculating dose and duration of viremia. These results aid in understanding the transmission dynamics of sylvatic DENV non-human primates, an issue of growing importance as dengue vaccines become available.

Keywords: Dengue virus; Non-human primates; Sylvatic dengue; Viral kinetics; Within-host dynamics.

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Figures

Figure 1
Figure 1
Flow Chart of Systematic Review
Figure 2
Figure 2. Days of Viremia for Primates
Figure shows the days of viremia for each non-human primate stratified by DENV serotype. Blue bars indicate DENV negative blood samples and red indicates positive samples. White indicates no samples. Data were sorted by DENV serotype, then by days of viremia.
Figure 3
Figure 3. Time to Detectable Viremia for DENV in Rhesus Macaque
Figure shows estimates of the time to detectable viremia for DENV-1–4 in Rhesus primates. Black lines indicate estimates from full dataset and light colored lines indicate bootstrap replicates. Grey lines indicate the 5th, 25th, 50th, 75th and 95th quantiles.
Figure 4
Figure 4. Duration of DENV Infection in Rhesus Macaque
Figure shows estimates of the duration of infection for DENV-1–4 in Rhesus primates. Black lines indicate estimates from full dataset and light colored lines indicate bootstrap replicates. Grey lines indicate the 5th, 25th, 50th, 75th and 95th quantiles.
See this image and copyright information in PMC

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