Recent achievments in the design and engineering of artificial metalloenzymes

Abstract

Herein, we highlight a selection of recent successes in the creation of artificial metalloenzymes. A particular emphasis is set on different anchoring methods to incorporate the abiotic metal cofactor within the host protein as well as promising strategies for the de novo design of artificial metalloenzymes. Both approaches yield promiscuous catalytic activities which expand the catalytic repertoire of biocatalysis and synthetic biology. Moreover, we summarize laboratory evolution protocols which have contributed to unravel the full potential of artificial metalloenzymes.