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. 2014 May;11(5):555-8.
doi: 10.1038/nmeth.2882. Epub 2014 Mar 9.

Single-molecule motions enable direct visualization of biomolecular interactions in solution

Quan Wang  1 , W E Moerner  2
Affiliations

Single-molecule motions enable direct visualization of biomolecular interactions in solution

Quan Wang et al. Nat Methods. 2014 May.

Abstract

Biomolecular interactions are generally accompanied by modifications in size and charge of biomolecules at the nanometer scale. Here we describe a single-molecule method to sense these changes in real time based on statistical learning of diffusive and electric field-induced motion parameters of a trapped molecule in solution. We demonstrate the approach by resolving a monomer-trimer mixture along a protein dissociation pathway and visualizing the binding-unbinding kinetics of a single DNA molecule.

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