Lipid-lowering effects of Pediococcus acidilactici M76 isolated from Korean traditional makgeolli in high fat diet-induced obese mice

Abstract

The effect of Pediococcus acidilactici M76 (lactic acid bacteria) isolated from makgeolli on mice fed a high fat diet was investigated to clarify the lipid lowering function. C57BL/6J male mice were randomly divided into a normal diet (ND) group, high fat diet (HD) group, HD plus Pediococcus acidilactici DSM 20284 reference strain (PR) group, and HD plus Pediococcus acidilactici M76 strain (PA) groups. The lyophilized PA and PR strain were dissolved in distilled water at a final concentration of 1.25 × 10⁹ cfu/mL and was given orally to animals at a dose of 4 mL/kg body weight for 12 weeks. The PA group had a lower final body weight, adipose tissue weight, and lipid profile than those in the HD group. Additionally, level of ACC, FAS and PPAR-γ, a key lipid synthesis enzyme, was markedly suppressed in the PA compared to those in the HD group. These data suggest that P. acidilactici M76 may exert a lipid-lowering effect in high fat diet-induced obese mice.

Figure 1

(A) Serum leptin and (B) insulin levels. Bars represent the mean ± SEM, n = 10 mice per group. Values with different superscripts are significantly different (p < 0.05) as assessed by one-way ANOVA and the Duncan’s multiple range test.

Figure 2

Hepatic mRNA expression of (A) ACC; (B) FAS; (C) PPAR-γ and (D) CPT-1. Bars represent the mean ± SEM, n = 10 mice per group. The mRNA levels of experimental groups were normalized to the level of the normal diet group. Values with different superscripts are significantly different (p < 0.05) as assessed by one-way ANOVA and the Duncan′s multiple range test.

Figure 3

(A) Serum aspartate aminotransferase and (B) alanine aminotransferase levels. Bars represent the mean ± SEM, n = 5 mice per group. Values with different superscripts are significantly different (p < 0.05) as assessed by one-way ANOVA and the Duncan’s multiple range test.