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. 1988 Jul;172(1):279-83.
doi: 10.1016/0003-2697(88)90443-5.

A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis

G J Wadsworth  1 , M G Redinbaugh, J G Scandalios
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A procedure for the small-scale isolation of plant RNA suitable for RNA blot analysis

G J Wadsworth et al. Anal Biochem. 1988 Jul.

Abstract

A small-scale method for the isolation of total RNA from plant tissue is described. The method provides RNA of suitable quantity and quality from 0.2 g fresh tissue for the detection of mRNA species by RNA blot analysis. The entire procedure is adapted to 1.5-ml microfuge tubes and takes less than 5 h. This method is well suited for the isolation of RNA from large numbers of samples or from samples of limited quantity.

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