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. 1988;54(218-219):175-9.

Simplified C-banding of mouse chromosomes

J R Edelman  1 , Y J Lin
Affiliations
  • PMID: 2461274

Simplified C-banding of mouse chromosomes

J R Edelman et al. Cytobios. 1988.

Abstract

Mouse bone marrow chromosomes incorporated with bromodeoxyuridine (BrdU) were differentially Giemsa stained after exposure to incandescent light in order to study sister chromatid exchange. Over-exposure of the chromosomes to incandescent light often resulted in the complete loss of sister chromatid differentiation, and it frequently resulted in the C-band formation. C-banding was also prominent with chromosomes which were not labelled with BrdU. The method utilized a 300 W, 130 V, inside-frosted incandescent bulb coupled with phosphate buffering at pH 6.8 and an exposure period of 2 h.

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