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. 2014 Mar 11:20:399-405.
doi: 10.12659/MSM.890528.

L-carnosine alters some hemorheologic and lipid peroxidation parameters in nephrectomized rats

Hande Yapislar  1 , Eylem Taskin  2
Affiliations

L-carnosine alters some hemorheologic and lipid peroxidation parameters in nephrectomized rats

Hande Yapislar et al. Med Sci Monit. .

Abstract

Background: Chronic kidney disease (CKD) is a major health problem worldwide. Oxidative stress is one of the mediators of this disease. Systemic complications of oxidative stress are involved in the pathogenesis of hypertension, endothelial dysfunction, shortened erythrocyte lifespan, deformability, and nitric oxide (NO) dysfunction. L-carnosine is known as an antioxidant. In this study, our aim was to investigate the effect of carnosine on hemorheologic and cardiovascular parameters in CKD-induced rats.

Material and methods: We used 4-month-old male Sprague-Dawley rats divided into 4 groups of 6 rats each. Three days after subtotal nephrectomy and sham operations, the surviving rats were divided into the 4 groups; 1) Sham (S), 2) Sham+Carnosine (S-C), 3) Subtotal nephrectomy (Nx), and 4) Subtotal nephrectomy + Carnosine (N-C). Carnosine was injected intraperitoneally (i.p.) (50 mg/kg) for 15 days. The control group received the same volume of physiological saline.

Results: In CKD rats, malondialdehyde (MDA) levels were increased, and NO and RBC deformability were decreased compared to Sham. Carnosine treatment decreased MDA levels, improved RBC (red blood cell) ability to deform, and increased NO levels. However, carnosine did not affect blood pressure levels in these rats.

Conclusions: We found that carnosine has beneficial effects on CKD in terms of lipid peroxidation and RBC deformability. Carnosine may have a healing effect in microcirculation level, but may not have any effect on systemic blood pressure in CKD-induced rats.

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Figures

Figure 1
Figure 1
Malondialdehyde levels of all groups. S – Sham-operated group, S-C – Carnosine-treated sham-operated group, Nx – Nephrectomy-operated group, N-C – Carnosine-treated nephrectomy-operated group. * p<0.05 when compared to S and ** p<0.05 when compared to N group.
Figure 2
Figure 2
Nitric oxide levels of all groups. S – Sham-operated group, S-C – Carnosine-treated sham-operated group, Nx – Nephrectomy-operated group, N-C – Carnosine-treated nephrectomy-operated group. * Compared to S group (p<0.05)
Figure 3
Figure 3
Elongation Index in all shear stress levels of all groups. S-C – Carnosine-treated sham-operated group, Nx – Nephrectomy-operated group, N-C – Carnosine-treated nephrectomy-operated group.
Figure 4
Figure 4
Blood pressure levels of all groups. S-C – Carnosine-treated sham-operated group, Nx – Nephrectomy-operated group, N-C – Carnosine-treated nephrectomy-operated group.
Figure 5
Figure 5
Heart rate of all groups. S-C – Carnosine-treated sham-operated group, Nx – Nephrectomy-operated group, N-C – Carnosine-treated nephrectomy-operated group. * p<0.05 Compared to S group.
See this image and copyright information in PMC

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