Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Dec;85(23):8939-42.
doi: 10.1073/pnas.85.23.8939.

Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex

R Jin  1 , K J Breslauer
Affiliations

Characterization of the minor groove environment in a drug-DNA complex: bisbenzimide bound to the poly[d(AT)].poly[d(AT)]duplex

R Jin et al. Proc Natl Acad Sci U S A. 1988 Dec.

Abstract

We compare the fluorescence properties of bisbenzimide (also known as Hoechst 33258) bound to the minor groove of the poly[d(AT)].poly[d(AT)] duplex with the corresponding fluorescence properties of bisbenzimide dissolved in neat organic solvents and mixed organic/aqueous solvents. Based on these comparisons, we conclude that the minor groove of the bisbenzimide-poly[d(AT)].poly[d(AT)] complex is quite nonpolar and exhibits a local dielectric constant of approximately 20 D. We discuss how this insight influences our understanding of the molecular forces that dictate and control the binding affinities and specificities of minor groove-directed DNA binding ligands.

PubMed Disclaimer

References

    1. Nucleic Acids Res. 1983 May 11;11(9):2857-70 - PubMed
    1. Nucleic Acids Res. 1985 Jul 11;13(13):4825-35 - PubMed
    1. Prog Biophys Mol Biol. 1986;47(1):31-112 - PubMed
    1. Nucleic Acids Res. 1988 Mar 25;16(6):2671-90 - PubMed
    1. Proc Natl Acad Sci U S A. 1987 Jul;84(13):4359-63 - PubMed

LinkOut - more resources