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. 1988 Dec;34(12):2528-32.

Concomitant purification of prostatic carcinoma tumor markers from human seminal fluid under nondenaturing conditions

D Rusciano  1 , A Berardi, C Ceccarini, B Terrana
Affiliations
  • PMID: 2461817

Concomitant purification of prostatic carcinoma tumor markers from human seminal fluid under nondenaturing conditions

D Rusciano et al. Clin Chem. 1988 Dec.

Abstract

We report a protocol for concomitant purification to homogeneity of both prostatic acid phosphatase [orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2] and prostate-specific antigen, from human seminal fluid. The method requires only two chromatographic steps: passage through an Affigel-Blue column and gel filtration HPLC. This is a fast, efficient procedure for purification of these two important tumor markers, which are specific for prostatic cancer.

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