Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Dec;85(24):9704-8.
doi: 10.1073/pnas.85.24.9704.

Identification, cloning, and characterization of an immune activation gene

M A Lipes  1 , M Napolitano, K T Jeang, N T Chang, W J Leonard
Affiliations

Identification, cloning, and characterization of an immune activation gene

M A Lipes et al. Proc Natl Acad Sci U S A. 1988 Dec.

Abstract

We have identified an immune activation gene, denoted Act-2, by differential hybridization screening of an activated T-cell library. The gene is induced rapidly after T-cell activation with phytohemagglutinin, B-cell activation with Staphylococcus aureus Cowan I, and monocyte activation with lipopolysaccharide. We have isolated a cDNA containing the full-length coding region. The deduced amino acid sequence predicts an open reading frame of 92 amino acids, including a very hydrophobic N terminus, which by weight matrix score is predicted to be a signal peptide. Using a baculovirus expression system, we have shown that this gene encodes a secreted product. It is therefore possible that Act-2 represents a newly discovered cytokine.

PubMed Disclaimer

References

    1. Mol Cell Biol. 1982 Feb;2(2):161-70 - PubMed
    1. Biochemistry. 1979 Nov 27;18(24):5294-9 - PubMed
    1. Anal Biochem. 1983 Jul 1;132(1):6-13 - PubMed
    1. Cell. 1983 Dec;35(3 Pt 2):603-10 - PubMed
    1. Nature. 1984 Oct 18-24;311(5987):626-31 - PubMed

Associated data