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. 1989 Jan 1;169(1):297-302.
doi: 10.1084/jem.169.1.297.

Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen

G Chimini  1 , P Pala, J Sire, B R Jordan, J L Maryanski
Affiliations

Recognition of oligonucleotide-encoded T cell epitopes introduced into a gene unrelated to the original antigen

G Chimini et al. J Exp Med. .

Abstract

We have previously demonstrated that H-2Kd-restricted CTL specific for HLA-CW3 or HLA-A24 can recognize synthetic peptides corresponding to residues 170-182 of the HLA molecules. Synthetic oligonucleotides encoding region 170-182 of CW3 or A24 were inserted into the influenza nucleoprotein (NP) gene. We demonstrate herein that P815 (H-2d) cells transfected with the NP-oligo recombinant genes are specifically lysed by HLA-specific Kd-restricted CTL clones. Our results imply that there must be a high degree of flexibility for the expression of T cell epitopes in different molecular contexts.

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