Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Nov 25;16(22):10751-64.
doi: 10.1093/nar/16.22.10751.

Analysis of potential expression of highly related members of the ribosomal protein L32 gene family

C M Jacks  1 , R E Biltz, P B Hackett
Affiliations
Free PMC article

Analysis of potential expression of highly related members of the ribosomal protein L32 gene family

C M Jacks et al. Nucleic Acids Res. .
Free PMC article

Abstract

The processed gene L32', a member of the mouse gene family for ribosomal protein L32, could encode a 135 amino acid protein nearly identical to L32. The 5'-flanking region of the gene contains CAAT and TATA sites at positions commonly found in expressed genes. The L32' gene lies within highly methylated, DNase I-insensitive chromatin of mouse L1210 cells. Although S1 nuclease digestion studies suggested that an L32' transcript might be produced, an oligonucleotide probe specific for L32' mRNA, and RNase digestion of a cRNA probe to L32', indicated fewer than 0.1 L32' transcripts/cell. These results demonstrate that extreme caution is required when measuring transcription from related genes.

PubMed Disclaimer

References

    1. Eur J Biochem. 1974 Sep 16;47(3):535-43 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Aug;81(16):5170-4 - PubMed
    1. Science. 1976 Sep 3;193(4256):848-56 - PubMed
    1. Nucleic Acids Res. 1976 Sep;3(9):2303-8 - PubMed
    1. Cell. 1976 Dec;9(4 PT 2):761-74 - PubMed

Publication types