Get your high-quality low-cost genome sequence

Abstract

The study of whole-genome sequences has become essential for almost all branches of biological research. Next-generation sequencing (NGS) has revolutionized the scalability, speed, and resolution of sequencing and brought genomic science within reach of academic laboratories that study non-model organisms. Here, we show that a high-quality draft genome of a eukaryote can be obtained at relatively low cost by exploiting a hybrid combination of sequencing strategies.

Keywords: eukaryote; fungal genome; genome structure; hybrid assembly; next-generation sequencing (NGS); repetitive element.