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. 1988 Oct;20(10):575-86.
doi: 10.1007/BF01002612.

A simple fixation and embedding method for use in hybridization histochemistry on plant tissues

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A simple fixation and embedding method for use in hybridization histochemistry on plant tissues

G I McFadden et al. Histochem J. 1988 Oct.

Abstract

A simple method for fixing and embedding plant tissue for hybridization histochemistry has been developed. Material is fixed with paraformaldehyde-glutaraldehyde by the phase-partition technique and embedded in LR Gold. The method preserves nucleic acids in situ. Double-stranded 32P-labelled DNA probes hybridized to complementary RNAs in thin and semithin sections. Biotinylated probes detected with immunogold markers have been used to localize transcripts at the electron microscopic level.

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References

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