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. 1988 Oct;20(10):575-86.
doi: 10.1007/BF01002612.

A simple fixation and embedding method for use in hybridization histochemistry on plant tissues

G I McFadden  1 , I Bonig, E C Cornish, A E Clarke
Affiliations

A simple fixation and embedding method for use in hybridization histochemistry on plant tissues

G I McFadden et al. Histochem J. 1988 Oct.

Abstract

A simple method for fixing and embedding plant tissue for hybridization histochemistry has been developed. Material is fixed with paraformaldehyde-glutaraldehyde by the phase-partition technique and embedded in LR Gold. The method preserves nucleic acids in situ. Double-stranded 32P-labelled DNA probes hybridized to complementary RNAs in thin and semithin sections. Biotinylated probes detected with immunogold markers have been used to localize transcripts at the electron microscopic level.

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References

    1. Histochem J. 1986 Nov-Dec;18(11-12):597-604 - PubMed
    1. Experientia. 1987 Jul 15;43(7):741-50 - PubMed
    1. J Histochem Cytochem. 1986 Nov;34(11):1389-98 - PubMed
    1. Planta. 1988 Dec;173(4):500-8 - PubMed
    1. J Histochem Cytochem. 1981 May;29(5):663-71 - PubMed

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