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. 2014 Mar 20:4:4416.
doi: 10.1038/srep04416.

Tea polyphenols induced apoptosis of breast cancer cells by suppressing the expression of Survivin

Xuesong Chen  1 , Yu Li  2 , Qiushi Lin  3 , Yan Wang  4 , Hong Sun  4 , Jian Wang  4 , Guoquan Cui  4 , Li Cai  5 , Xiaoqun Dong  6
Affiliations

Tea polyphenols induced apoptosis of breast cancer cells by suppressing the expression of Survivin

Xuesong Chen et al. Sci Rep. .

Abstract

To study the mechanism of tea polyphenols (TP)-induced apoptosis of breast cancer cells. Proliferation of MCF-7 and SK-BR-3 cells was evaluated by MTT assays. Cellular ultrastructure was examined by electron microscopy. Apoptosis was detected by TUNEL. PCNA、 Cyclin D1、 Cyclin E and Survivin expression was measured by Western blot. Cell proliferation was significantly inhibited by TP. Spindle and round cells were loosely distributed with increased particles after TP treatment. Increased cell size, frequent nuclear atypia and a collapse of apoptosis were observed. The nucleus was pushed towards one side, while the cytoplasm was rich in free ribosome. The membrane of mitochondria was thickening, and the cell apoptotic body was observed. TP treated cells experienced significantly enhanced apoptosis compared with 5-Fu treated or control groups. The expression of survivin was downregulated by TP. To conclude, TP can inhibit cell growth and induce apoptosis through downregulating the expression of survivin in breast cancer.

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Figures

Figure 1
Figure 1. Effects of TP on cell proliferation.
Inhibition rate was dramatically increased as a dose-dependent manner in response to TP treatment in (A) SK-BR-3 and (B) MCF-7 cells.
Figure 2
Figure 2. Effect of TP on cell morphology.
(A) Morphological changes of MCF-7 cells after 5-Fu or TP treatment. (B) Ultramicrostructure changes of MCF-7 cells induced by 5-Fu or TP.
Figure 3
Figure 3. TUNEL staining of MCF-7 cells treated by 5-Fu or TP (*P < 0.05).
Figure 4
Figure 4. Expression of Survivin in SK-BR-3 and MCF-7 cells after TP treatment (*P < 0.05).
C: control; T: TP.
Figure 5
Figure 5. Expression of PCNA, Cyclin D1, and Cyclin E in SK-BR-3 and MCF-7 cells after TP treatment.
Figure 6
Figure 6. Effects of TP on the growth of subcutaneous xenograft tumors derived from MCF-7 cells in nude mice (*P < 0.05).
(A) Subcutaneous xenograft tumor-bearing nude mice treated with TP for 38 days and tumor volumes at various time points after tumor implantation. (B) Expression of survivin in MCF-7 cells treated with TP for 38 days.
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