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Comment
. 2014 Mar 18;33(6):536-9.
doi: 10.1002/embj.201384895.

Antisense piRNA amplification, but not piRNA production or nuage assembly, requires the Tudor-domain protein Qin

Zhao Zhang  1 , Birgit S Koppetsch, Jie Wang, Cindy Tipping, Zhiping Weng, William E Theurkauf, Phillip D Zamore
Affiliations
Comment

Antisense piRNA amplification, but not piRNA production or nuage assembly, requires the Tudor-domain protein Qin

Zhao Zhang et al. EMBO J. .
No abstract available

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Figures

Figure 1
Figure 1
Without Qin, Ago3, Aub and Vasa still reside in nurse cell nuage.

  1. RNA-seq data for wild-type and qin mutant ovaries.

  2. Ago3 and Aub immunostaining or live EGFP-Vasa image in qin mutants. EGFP-Vasa fusion protein was expressed from a transgene using the vasa promoter.

Figure 2
Figure 2
piRNA abundance and Ping-Pong efficiency are unaltered in qin mutant ovaries.

  1. piRNA length distribution. Blue, sense piRNAs; red, antisense.

  2. Box plots reporting the change in abundance of all piRNAs mapping to transposons.

  3. qin1/Df, qin2/Df and qinkumo/Df, but not qinkumo/qinkumo, affect piRNA production similarly. Group 1: transposon families with piRNAs amplified by the Ping-Pong pathway and more antisense piRNAs bound to Aub and more sense piRNAs bound to Ago3. Group 2: transposon families with piRNAs amplified by the Ping-Pong pathway and more sense piRNAs bound to Aub and more antisense piRNAs bound to Ago3. Group 3: transposon families expressed in the somatic follicle cells, predominantly antisense primary piRNAs, and little Ping-Pong amplification.

  4. Box plots reporting Ping-Pong Z-score by transposon family. Z-score = 1.96 corresponds to P-value = 0.05.

See this image and copyright information in PMC

Comment in

  • Response to Zhang et al.
    Anand A, Kai T. Anand A, et al. EMBO J. 2014 Mar 18;33(6):540-1. doi: 10.1002/embj.201387548. EMBO J. 2014. PMID: 24652837 Free PMC article. No abstract available.

Comment on

References

    1. Anand A, Kai T. The tudor domain protein kumo is required to assemble the nuage and to generate germline piRNAs in Drosophila. EMBO J. 2012;31:870–882. - PMC - PubMed
    1. Brennecke J, Aravin AA, Stark A, Dus M, Kellis M, Sachidanandam R, Hannon GJ. Discrete small RNA-generating loci as master regulators of transposon activity in Drosophila. Cell. 2007;128:1089–1103. - PubMed
    1. Zhang Z, Xu J, Koppetsch BS, Wang J, Tipping C, Ma S, Weng Z, Theurkauf WE, Zamore PD. Heterotypic piRNA ping-pong requires Qin, a protein with both E3-ligase and tudor domains. Mol Cell. 2011;44:572–584. - PMC - PubMed

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