A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR

Justin Hardick¹, Sufyan Sadiq², Elizabeth Perelstein², Stephen Peterson², Richard Rothman³, Charlotte A Gaydos³

Affiliations

¹ Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD, USA. Electronic address: Jhardic1@jhmi.edu.
² Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD, USA.
³ Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD, USA; Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD, USA.

PMID: 24657170
PMCID: PMC4557781
DOI: 10.1016/j.diagmicrobio.2014.02.009

Comparative Study

A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR

Justin Hardick et al. Diagn Microbiol Infect Dis. 2014 Jun.

. 2014 Jun;79(2):187-9.

doi: 10.1016/j.diagmicrobio.2014.02.009. Epub 2014 Feb 24.

Authors

Justin Hardick¹, Sufyan Sadiq², Elizabeth Perelstein², Stephen Peterson², Richard Rothman³, Charlotte A Gaydos³

Affiliations

¹ Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD, USA. Electronic address: Jhardic1@jhmi.edu.
² Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD, USA.
³ Johns Hopkins University School of Medicine, Division of Infectious Diseases, Baltimore, MD, USA; Johns Hopkins University, Department of Emergency Medicine, Baltimore, MD, USA.

PMID: 24657170
PMCID: PMC4557781
DOI: 10.1016/j.diagmicrobio.2014.02.009

Abstract

Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, and specificity. Sensitivity and specificity were 91% (111/122) and 95.1% (116/122), respectively. Sensitivity by virus, except parainfluenza, was 92.9-100%, and specificity was 99-100%.

Keywords: DFA; RT-PCR/ESI-MS; Respiratory virus diagnostics; xTAG RVP.

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References

1. Chen K.F., Rothman R.E., Ramachandran P., Blyn L., Sampath R., Ecker D.J. Rapid identification viruses from nasal pharyngeal aspirates in acute viral respiratory infections by RT-PCR and electrospray ionization mass spectrometry. J Virol Methods. 2011;173:60–66. - PMC - PubMed
1. Chen K.F., Blyn L., Rothman R.E., Ramachandran P., Valsamakis A., Ecker D. Reverse transcription polymerase chain reaction and electrospray ionization mass spectrometry for identifying acute viral upper respiratory tract infections. Diagn Microbiol Infect Dis. 2011;69:179–186. - PMC - PubMed
1. Deyde V.M., Sampath R., Garten R.J., Blair P.J., Myers C.A., Massire C. Genomic signature-based identification of influenza A viruses using RT-PCR/electro-spray ionization mass spectrometry (ESI-MS) technology. PLoS One. 2010;5:e13293. - PMC - PubMed
1. Deyde V.M., Sampath R., Gubareva L.V. RT-PCR/electrospray ionization mass spectrometry approach in detection and characterization of influenza viruses. Expert Rev Mol Diagn. 2011;11:41–52. - PubMed
1. Forman M.S., Advani S., Newman C., Gaydos C.A., Milstone A.M., Valsamakis A. Diagnostic performance of two highly multiplexed respiratory virus assays in a pediatric cohort. J Clin Virol. 2012;55:168–172. - PMC - PubMed

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A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR

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A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR

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