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Comparative Study
. 2014 Jun;79(2):187-9.
doi: 10.1016/j.diagmicrobio.2014.02.009. Epub 2014 Feb 24.

A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR

Justin Hardick  1 , Sufyan Sadiq  2 , Elizabeth Perelstein  2 , Stephen Peterson  2 , Richard Rothman  3 , Charlotte A Gaydos  3
Affiliations
Comparative Study

A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR

Justin Hardick et al. Diagn Microbiol Infect Dis. 2014 Jun.

Abstract

Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, and specificity. Sensitivity and specificity were 91% (111/122) and 95.1% (116/122), respectively. Sensitivity by virus, except parainfluenza, was 92.9-100%, and specificity was 99-100%.

Keywords: DFA; RT-PCR/ESI-MS; Respiratory virus diagnostics; xTAG RVP.

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References

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