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. 2014:2014:162517.
doi: 10.1155/2014/162517. Epub 2014 Feb 11.

Serum omentin-1 as a disease activity marker for Crohn's disease

Yan Lu  1 , Li Zhou  2 , Lifeng Liu  1 , Yan Feng  3 , Li Lu  4 , Xiaoyan Ren  5 , Xinqian Dong  6 , Weiwei Sang  1
Affiliations

Serum omentin-1 as a disease activity marker for Crohn's disease

Yan Lu et al. Dis Markers. 2014.

Abstract

Background and aim: It remains challenging to determine the inflammatory activity in Crohn's disease (CD) for lack of specific laboratory markers. Recent studies suggest that serum omentin-1 is associated with inflammatory response. We aimed to assess the potential of serum omentin-1 as a marker of disease activity in CD patients.

Methods: Serum omentin-1 concentrations were determined by enzyme-linked immunosorbent assay (ELISA) in patients with CD (n = 240), functional gastrointestinal disorders (FGDs, n = 120), and healthy controls (HC, n = 60) and evaluated for correlation with disease activity. Expression of omentin-1 in colonic tissues from patients with CD was also analyzed by real-time PCR and Western blotting. Serum omentin-1 levels as an activity index were evaluated using a receiver operating characteristic (ROC) curve.

Results: Serum omentin-1 concentrations were significantly decreased in active CD patients compared with patients in remission, FGDs, and HC (all P < 0.001). Expression of omentin-1 was decreased at mRNA and protein levels in inflamed colonic tissues in active CD than that in noninflamed colonic tissues. Serum omentin-1 levels were negatively correlated with disease activity in CD, better than C-reactive protein (CRP).

Conclusion: Our results indicate that serum and colonic omentin-1 expressions are decreased in active CD patients. The correlation of serum omentin-1 with disease activity in CD is superior to that of CRP. Serum omentin-1 is a potential marker for CD disease activity.

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Figures

Figure 1
Figure 1
Serum omentin-1 concentrations in the healthy controls (HC, n = 60), functional gastrointestinal disorders (FGDs, n = 120), active Crohn's disease (CD, n = 112), and CD in remission (n = 128). # P < 0.01 versus active CD, *P < 0.01 versus active CD, **P < 0.01 versus active CD.
Figure 2
Figure 2
Serum omentin-1 levels are correlated with disease activity in Crohn's disease (CD) patients. A negative correlation was observed between serum omentin-1 level and CDAI (r = −0.65, P < 0.0001) (a). There was a positive correlation observed between CRP and CDAI (r = 0.51, P < 0.0001) (b).
Figure 3
Figure 3
Expression of omentin-1 was decreased in inflamed Crohn's disease (CD) colons. qRT-PCR results showed that omentin-1 expression in colon tissues was decreased in inflamed sites of active CD patients compared with noninflamed colon tissues (a). Western blot analysis confirmed the lower omentin-1 in inflamed colon tissues than that in noninflamed sites (b).
Figure 4
Figure 4
Serum omentin-1 levels as an activity index in Crohn's disease (CD) patients. The cutoff value of serum omentin-1 levels was 303.43 ng/mL (yielding sensitivity and specificity values of 74.5% and 84.0%). In contrast, when the cutoff value of CRP levels was set at 3.4 mg/mL, the sensitivity was 63.0% and the specificity was 72.3%. We found that the area under the ROC curve (AUC) for serum omentin-1 levels was 0.87, whereas the AUC for CRP levels was 0.76.
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