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. 2014 Feb;4(1):37-45.
doi: 10.1007/s13659-013-0001-3. Epub 2014 Feb 18.

Isolation of Microcystins from the Cyanobacterium Planktothrix rubescens Strain No80

Timo H J Niedermeyer  1 , Peter Schmieder  2 , Rainer Kurmayer  3
Affiliations

Isolation of Microcystins from the Cyanobacterium Planktothrix rubescens Strain No80

Timo H J Niedermeyer et al. Nat Prod Bioprospect. 2014 Feb.

Abstract

Three minor microcystins have been isolated from a Planktothrix rubescens strain. Their structures have been elucidated by one- and two-dimensional NMR spectroscopy and high-resolution tandem mass spectrometry as the compounds [Asp(3),(E)-Dhb(7)]MC-LY (1), [Asp(3),(E)-Dhb(7)]MC-HtyW (2), and [Asp(3),(E)-Dhb(7)]MC-LW (3). The amino acids found at the variable positions 2 and 4 of the microcystin core structure are in accordance with the predicted amino acid substrate activation selectivities of the non-ribosomal peptide synthetases McyA and McyB described earlier for this strain. All structural microcystin variants produced by this strain were shown to inhibit protein phosphatase 1 in the nanomolar range.

Keywords: Cyanobacteria; Microcystins; Planktothrix; Protein phosphatase inhibition.

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Figures

Fig. 1
Fig. 1
Interlaced b-ions observed in the tandem MS spectrum of 3 (for details on the observed fragments see ESM)
See this image and copyright information in PMC

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