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. 2014 Feb;32(1):27-31.
doi: 10.7518/hxkq.2014.01.007.

[Preparation of sodium alginate-nanohydroxyapatite composite material for bone repair and its biocompatibility]

[Article in Chinese]
Yanmei Wang, Jiacai He, Quanli Li, Jijia Shen

[Preparation of sodium alginate-nanohydroxyapatite composite material for bone repair and its biocompatibility]

[Article in Chinese]
Yanmei Wang et al. Hua Xi Kou Qiang Yi Xue Za Zhi. 2014 Feb.

Abstract
in English, Chinese

Objective: To prepare sodium alginate-nanohydroxyapatite composite material and to explore its feasibility as a bone repair material.

Methods: Sodium alginate-nanohydroxyapatite composite material was prepared using chemical cross-linking and freeze-drying technology. The composite was characterized by X-ray diffraction (XRD) and scanning electron microscope (SEM) and its porosity was measured by liquid displacement method. The fifth passage of bone marrow stromal stem cells (BMSCs) were incubated on the composite material and then growth was observed by inverted microscope and SEM. BMSCs were cultured with liquid extracts of the material, methyl thiazolyl tetrazolium (MTT) assay was used to calculate the relative growth rate (RGR) on 1, 3, 5 d and to evaluate the cytotoxicity. Fresh dog blood was added into the liquid extracts to conduct hemolysis test, the spectrophotometer was used to determine the optical density (OD) and to calculate the hemolysis rate.

Results: Sodium alginate-nanohydroxyapatite composite material displayed porosity, the porous pore rate was (88.6 +/- 4.5)%. BMSCs showed full stretching and vigorous growth under inverted microscope and SEM. BMSCs cultured with liquid extracts of the material had good activities. The toxicity of composite material was graded as 1. Hemolysis test results showed that the hemolysis rate of the composite material was 1.28%, thus meeting the requirement of medical biomaterials.

Conclusion: The composite material fabricated in this study has high porosity and good biocompatibility.

目的: 制备海藻酸钙-纳米羟磷灰石复合材料，探讨其作为骨修复材料的可行性。

方法: 应用化学交联及冷冻干燥技术制备海藻酸钙-纳米羟磷灰石复合材料，通过扫描电镜（SEM）和X光衍射（XRD）对材料性能进行表征，液体置换法测定孔隙率。将第5代骨髓基质干细胞（BMSCs）接种至材料上，倒置显微镜及SEM观察细胞生长情况。材料浸提液与BMSCs共培养，甲基噻唑基四唑（MTT）法检测接种后1、3、5 d的相对增殖率（RGR），并评估毒性分级。新鲜犬血加入材料浸提液进行溶血试验，分光光度计测定光密度值并计算溶血率。

结果: 制备的海藻酸钙-纳米羟磷灰石复合材料具有多孔性，孔隙率达（88.6±4.5）%。倒置显微镜及SEM观察可见，细胞在材料周围及表面伸展充分、生长旺盛。材料浸提液培养的细胞具有较好的活力，其毒性分级为1级。溶血试验结果表明复合材料的溶血率为1.28%，满足医用生物材料的要求。

结论: 本研究制备的复合材料具有较高的孔隙率和良好的生物相容性。

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Figures

图 1
图 1. 复合材料的观察结果 SEM × 2 000
Fig 1 Observation of CM SEM × 2 000
图 2
图 2. ALG、nHA及CM的X线衍射图谱
Fig 2 XRD patterns of ALG, nHA and CM
图 3
图 3. BMSCs在材料上生长5 d的形态学观察
Fig 3 Morphology of BMSCs attached to composite in fifth days 左：倒置显微镜 × 100；右：SEM × 250。
图 4
图 4. BMSCs在各组培养介质中生长1 d时的形态观察 倒置显微镜 × 40
Fig 4 Morphology of BMSCs in each culture medium in first day inverted microscope × 40 左：A组；中：B组；右：C组。
图 5
图 5. 溶血试验样本大体观
Fig 5 General observation of samples after hemolysis test 图中从左至右分别是生理盐水组、浸提液组、双蒸水组。
See this image and copyright information in PMC

References

    1. Eiden-Assmann S, Viertelhaus M, Heiss A, et al. The influence of amino acids on the biomineralization of hydroxyapatite in gelatin[J] J Inorg Biochem. 2002;91(3):481–486. - PubMed
    1. Chang MC, Ko CC, Douglas WH. Preparation of hydroxyapatite-gelatin nanocomposite[J] Biomaterials. 2003;24(17):2853–2862. - PubMed
    1. Kim HW, Kim HE, Salih V. Stimulation of osteoblast responses to biomimetic nanocomposites of gelatin-hydroxyapatite for tissue engineering scaffolds[J] Biomaterials. 2005;26(25):5221–5230. - PubMed
    1. 张 金鹏, 汤 逊. 纳米羟基磷灰石复合支架材料的研究与应用[J] 中国组织工程研究与临床康复. 2011;15(42):7887–7891.
    1. 张 维茹. 关于滤材孔隙率的探讨[J] 天津造纸. 1997;19(4):19–22.

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