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. 2014 Mar 26:14:43.
doi: 10.1186/1471-2288-14-43.

SYRCLE's risk of bias tool for animal studies

Carlijn R Hooijmans  1 , Maroeska M Rovers, Rob B M de Vries, Marlies Leenaars, Merel Ritskes-Hoitinga, Miranda W Langendam
Affiliations

SYRCLE's risk of bias tool for animal studies

Carlijn R Hooijmans et al. BMC Med Res Methodol. .

Abstract

Background: Systematic Reviews (SRs) of experimental animal studies are not yet common practice, but awareness of the merits of conducting such SRs is steadily increasing. As animal intervention studies differ from randomized clinical trials (RCT) in many aspects, the methodology for SRs of clinical trials needs to be adapted and optimized for animal intervention studies. The Cochrane Collaboration developed a Risk of Bias (RoB) tool to establish consistency and avoid discrepancies in assessing the methodological quality of RCTs. A similar initiative is warranted in the field of animal experimentation.

Methods: We provide an RoB tool for animal intervention studies (SYRCLE's RoB tool). This tool is based on the Cochrane RoB tool and has been adjusted for aspects of bias that play a specific role in animal intervention studies. To enhance transparency and applicability, we formulated signalling questions to facilitate judgment.

Results: The resulting RoB tool for animal studies contains 10 entries. These entries are related to selection bias, performance bias, detection bias, attrition bias, reporting bias and other biases. Half these items are in agreement with the items in the Cochrane RoB tool. Most of the variations between the two tools are due to differences in design between RCTs and animal studies. Shortcomings in, or unfamiliarity with, specific aspects of experimental design of animal studies compared to clinical studies also play a role.

Conclusions: SYRCLE's RoB tool is an adapted version of the Cochrane RoB tool. Widespread adoption and implementation of this tool will facilitate and improve critical appraisal of evidence from animal studies. This may subsequently enhance the efficiency of translating animal research into clinical practice and increase awareness of the necessity of improving the methodological quality of animal studies.

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References

    1. Sandercock P, Roberts I. Systematic reviews of animal experiments. Lancet. 2002;360(9333):586. doi: 10.1016/S0140-6736(02)09812-4. - DOI - PubMed
    1. Hooijmans CR, Rovers M, de Vries RB, Leenaars M, Ritskes-Hoitinga M. An initiative to facilitate well-informed decision-making in laboratory animal research: report of the First International Symposium on Systematic Reviews in Laboratory Animal Science. Lab Anim. 2012;46(4):356–357. doi: 10.1258/la.2012.012052. - DOI - PubMed
    1. Muhlhausler BS, Bloomfield FH, Gillman MW. Whole animal experiments should be more like human randomized controlled trials. PLoS Biol. 2013;11(2):e1001481. doi: 10.1371/journal.pbio.1001481. - DOI - PMC - PubMed
    1. Chalmers TC, Celano P, Sacks HS, Smith H Jr. Bias in treatment assignment in controlled clinical trials. N Engl J Med. 1983;309(22):1358–1361. doi: 10.1056/NEJM198312013092204. - DOI - PubMed
    1. Macleod MR, Fisher M, O’Collins V, Sena ES, Dirnagl U, Bath PM, Buchan A, van der Worp HB, Traystman R, Minematsu K, Donnan GA, Howells DW. Good laboratory practice: preventing introduction of bias at the bench. Stroke. 2009;40(3):e50–e52. doi: 10.1161/STROKEAHA.108.525386. - DOI - PubMed

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