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Comparative Study
. 1989 Mar 31;118(2):187-92.
doi: 10.1016/0022-1759(89)90005-7.

Quantitative enzyme immunoassay for human granulocyte colony stimulating factor (G-CSF)

H Motojima  1 , T Kobayashi, M Shimane, S Kamachi, M Fukushima
Affiliations
Comparative Study

Quantitative enzyme immunoassay for human granulocyte colony stimulating factor (G-CSF)

H Motojima et al. J Immunol Methods. .

Abstract

Human granulocyte colony stimulating factor (G-CSF) was detected at the pM level by a simple sequential sandwich enzyme immunoassay. The polyclonal antiserum obtained by immunizing rabbits with recombinant G-CSF did not recognize other colony stimulating factors. The EIA developed gave satisfactory reproducibility as judged by an intra-assay precision of 4.3-6.2% and an interassay precision of 6.2-9.0%. Results from this assay procedure correlated well with those of a bioassay. The method could be performed within 6 h.

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