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. 2014 Jan;6(1):46-50.
doi: 10.4103/0974-7796.127029.

The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats

Ahmet A Sancaktutar  1 , Mehmet N Bodakci  1 , Namık K Hatipoglu  1 , Haluk Soylemez  1 , Kemal Basarılı  2 , Gul Turkcu  3
Affiliations

The protective effects of pomegranate extracts against renal ischemia-reperfusion injury in male rats

Ahmet A Sancaktutar et al. Urol Ann. 2014 Jan.

Abstract

Aim: To evaluate the possible protective effect of pomegranate extract (PE) on rats following renal ischemia-reperfusion (I/R) injury.

Materials and methods: Twenty-four Wistar rats were divided into three groups. Sham group underwent laparotomy then waited for 45 minutes without ischemia. I/R group were subjected to left renal ischemia for 45 minutes followed by 60 minutes of reperfusion. I/R + PE group were subjected to the same renal I/R as the I/R group were also given 225 mg/kg PE peroral 30 minutes prior to the ischemia. Malondialdehyde (MDA), total antioxidant capacity (TAC), total oxidant status (TOS), and oxidative stress index (OSI) were determined on the blood samples and kidney tissues. Histopathological analyses were conducted on the kidney tissues.

Results: Serum TAC levels were significantly decreased in I/R group when compared with S group (P = 0.001). Serum MDA levels were increased in I/R group; however, it was not statistically significant. In rat kidney tissues, TOS levels and OSI index were significantly increased after I/R injury, while TAC levels were decreased. In I/R + PE group, PE reversed the negative effects of I/R injury. PE pretreatment was effective in decreasing tubular necrosis score.

Conclusion: PE pretreatment ameliorated the oxidative damage and histopathological changes occurring following renal I/R injury.

Keywords: Antioxidant; ischemia-reperfusion; kidney; pomegranate extract; rat.

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Conflict of interest statement

Conflict of Interest: None

Figures

Figure 1
Figure 1
(a) Sham group. Normal histomorphological features of the renal tubules. Mild tubular cell swelling is seen in a few tubular structures (H and E stain, ×200). (b) Ischemia reperfusion group. Note tubular cell swelling, brush border loss, and nuclear condensation with nuclear losses (H and E stain, ×200). (c) Ischemia reperfusion+Pomegranate extract group. Note tubular cell swelling, slight brush border loss, and a few instances of nuclear condensation with some nuclear losses (H and E stain, ×200)
Figure 2
Figure 2
Histopathological evaluation of renal tissue for each group. Scores were significantly lower in IR+PE group than in the I/R group. (Pearson Chi-Square test, P = 0,024). I/R: Ischemia/reperfusion, PE: Pomegranate extract
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