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. 2014 Mar 25;15(4):5263-76.
doi: 10.3390/ijms15045263.

Endothelialization of novel magnesium-rare earth alloys with fluoride and collagen coating

Nan Zhao  1 , Benjamin Workman  2 , Donghui Zhu  3
Affiliations

Endothelialization of novel magnesium-rare earth alloys with fluoride and collagen coating

Nan Zhao et al. Int J Mol Sci. .

Abstract

Magnesium (Mg) alloys are promising scaffolds for the next generation of cardiovascular stents because of their better biocompatibility and biodegradation compared to traditional metals. However, insufficient mechanical strength and high degradation rate are still the two main limitations for Mg materials. Hydrofluoric acid (HF) treatment and collagen coating were used in this research to improve the endothelialization of two rare earth-based Mg alloys. Results demonstrated that a nanoporous film structure of fluoride with thickness of ~20 µm was formed on the Mg material surface, which improved the corrosion resistance. Primary human coronary artery endothelial cells (HCAECs) had much better attachment, spreading, growth and proliferation (the process of endothelialization) on HF-treated Mg materials compared to bare- or collagen-coated ones.

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Figures

Figure 1.
Figure 1.
SEM images of type I collagen coating morphologies. All three materials were incubated with 50 μL 200 μg/mL collagen solutions in DPBS for 2 h.
Figure 2.
Figure 2.
SEM images of collagen on pure Mg. Collagen stock solution was diluted by R1 (Left) and R2 (Right) extract solution and then coated on the pure Mg surface.
Figure 3.
Figure 3.
SEM images of fluoride coating morphologies. All the materials were treated with HF solution for 3 days.
Figure 4.
Figure 4.
EDS Mapping for cross section of fluoride coating. First row: SEM photos displaying cross sections of magnesium fluoride coating in epoxide resin; Second row: magnesium (Mg, green); Third row: fluorine (F, red). Yellow lines indicate the thickness of fluoride coating (Scale bar: 10 μm).
Figure 5.
Figure 5.
DC Polarization curves of three materials in HBS.
Figure 6.
Figure 6.
Endothelial cells cultured on bare Mg, collagen coated Mg, and HF treated Mg from day 1 to day 3 (Scale bar: 10 μm).
Figure 7.
Figure 7.
Endothelial cells cultured on bare R1, collagen coated R1 and HF treated R1 alloys from day 1 to day 3 (Scale bar: 10 μm).
Figure 8.
Figure 8.
Endothelial cells cultured on bare R2, collagen coated R2 and HF treated R2 alloys from day 1 to day 3. (Scale bar: 10 μm).
See this image and copyright information in PMC

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