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. 2014 Mar 17:5:100.
doi: 10.3389/fmicb.2014.00100. eCollection 2014.

Identification of carotenoids from the extremely halophilic archaeon Haloarcula japonica

Rie Yatsunami  1 , Ai Ando  1 , Ying Yang  1 , Shinichi Takaichi  2 , Masahiro Kohno  1 , Yuriko Matsumura  1 , Hiroshi Ikeda  1 , Toshiaki Fukui  1 , Kaoru Nakasone  3 , Nobuyuki Fujita  4 , Mitsuo Sekine  4 , Tomonori Takashina  5 , Satoshi Nakamura  1
Affiliations

Identification of carotenoids from the extremely halophilic archaeon Haloarcula japonica

Rie Yatsunami et al. Front Microbiol. .

Abstract

The carotenoids produced by extremely halophilic archaeon Haloarcula japonica were extracted and identified by their chemical, chromatographic, and spectroscopic characteristics (UV-Vis and mass spectrometry). The composition (mol%) was 68.1% bacterioruberin, 22.5% monoanhydrobacterioruberin, 9.3% bisanhydrobacterioruberin, <0.1% isopentenyldehydrorhodopin, and trace amounts of lycopene and phytoene. The in vitro scavenging capacity of a carotenoid, bacterioruberin, extracted from Haloarcula japonica cells against 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals was evaluated. The antioxidant capacity of bacterioruberin was much higher than that of β -carotene.

Keywords: C50 carotenoid; Haloarcula japonica; antioxidant capacity; bacterioruberin; extremely halophilic archaeon.

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Figures

FIGURE 1
FIGURE 1
Elution profile of pigments extracted from Haloarcula japonica. The HPLC system consisted of a reversed-phase μBondapack C18 column. Absorbance at 490 nm is shown. Peak identification is revealed in Table 1. St: internal standard of ethyl β-apo-8′-carotenoate.
FIGURE 2
FIGURE 2
Structure of carotenoids produced by Haloarcula japonica.
FIGURE 3
FIGURE 3
Radical scavenging activities of BR and β-carotene against DPPH radicals. White bars, β-carotene; Black bars, BR.
See this image and copyright information in PMC

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