Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014 Mar 28:11:15.
doi: 10.1186/1743-7075-11-15. eCollection 2014.

Coacervate whey protein improves inflammatory milieu in mice fed with high-fat diet

Mayara Franzoi Moreno  1 , Gabriel Inácio de Morais Honorato de Souza  1 , Ana Claudia Losinskas Hachul  1 , Bruno Dos Santos  1 , Marcos Hiromu Okuda  1 , Nelson Inácio Pinto Neto  1 , Valter Tadeu Boldarine  1 , Elisa Esposito  2 , Eliane Beraldi Ribeiro  1 , Claudia Maria da Penha Oller do Nascimento  1 , Aline de Piano Ganen  1 , Lila Missae Oyama  1
Affiliations

Coacervate whey protein improves inflammatory milieu in mice fed with high-fat diet

Mayara Franzoi Moreno et al. Nutr Metab (Lond). .

Abstract

Background: Functional foods with bioactive properties may help in treat obesity, as they can lead to a decreased risks of inflammatory diseases. The aim of this study was to investigate the effects of chitosan coacervate whey protein on the proinflammatory processes in mice fed with high-fat diet.

Methods: Mice were divided into two groups receiving either a normolipidic or high-fat diet; the animals in each of the two diet groups were given a diet supplement of either coacervate (gavage, 36 mg protein/kg of body weight) or tap water for four weeks [groups: normolipidic diet plus water (C); normolipidic diet and coacervate (CC); high-fat diet and water (H); and high-fat diet and coacervate (HC)].

Results: The high-fat diet promoted inflammation, possibly by decreased adiponectin/sum of adipose tissues ratio and increased phosphorylation of NF-κB p50. In HC we observed a positive correlation between IL-10 and TNF-α in mesenteric adipose tissue, retroperitoneal adipose tissue and liver tissue. We also observed a positive correlation between lipopolisaccharide with IL-10 in the liver tissue.

Conclusions: High-fat diet treatment promoted metabolic alterations and inflammation, and chitosan coacervate whey protein modulated inflammatory milieu.

Keywords: Cytokines; IL-10; Mesenteric adipose tissue.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Average body mass (g) per week in the experimental groups treated with high-fat or control diet (cycle I); and high-fat diet or control associated with gavage of water or coacervate (cycle II); (n = 9-14). *different from C (p = 0.04); †different from C, CC and H (p < 0.02).
Figure 2
Figure 2
Western blot analysis of p-pNF-kBp65, p-NF-kBp50, p-IκB, TLR4, GPx-3, SOD-1 protein in MES adipose tissue in different groups. Data are mean ± SEM (n = 4–9); *different from C (p ≤ 0.01).
Figure 3
Figure 3
Positive correlation between IL-10 and TNF-α in liver, mesenteric adipose tissue, and retroperitoneal adipose tissue. Rows represent tissue, and columns groups (n = 7-9).
Figure 4
Figure 4
Correlation between LPS and IL-10 in liver (n = 6-8).
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Furukawa S, Fujita T, Shimabukuro M, Iwaki M, Yamada Y, Nakajima Y, Nakayama O, Makishima M, Matsuda M, Shimomura I. Increased oxidative stress in obesity and its impact on metabolic syndrome. J Clin Invest. 2004;114:1752–1761. doi: 10.1172/JCI21625. - DOI - PMC - PubMed
    1. Kasbia GS. Functional foods and nutraceuticals in the management of obesity. Nutr Food Sci. 2005;35:344–352. doi: 10.1108/00346650510625557. - DOI
    1. Kent KD, Harper WJ, Bomser JA. Effect of whey protein isolate on intracellular glutathione and oxidant-induced cell death in human prostate epithelial cells. Toxicol In Vitro. 2003;17(1):27–33. doi: 10.1016/S0887-2333(02)00119-4. - DOI - PubMed
    1. Dziuba M, Darewicz M. Food Proteins as precursors of bioactive peptides - classification into families. Food Sci Technol Int. 2007;13:393–404. doi: 10.1177/1082013208085933. - DOI
    1. Aimutis WR. Bioactive properties of milk proteins with particular focus on anticariogenesis. J Nutr. 2004;134:989S–995S. - PubMed