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. 2014 Mar 28:11:15.
doi: 10.1186/1743-7075-11-15. eCollection 2014.

Coacervate whey protein improves inflammatory milieu in mice fed with high-fat diet

Mayara Franzoi Moreno  1 , Gabriel Inácio de Morais Honorato de Souza  1 , Ana Claudia Losinskas Hachul  1 , Bruno Dos Santos  1 , Marcos Hiromu Okuda  1 , Nelson Inácio Pinto Neto  1 , Valter Tadeu Boldarine  1 , Elisa Esposito  2 , Eliane Beraldi Ribeiro  1 , Claudia Maria da Penha Oller do Nascimento  1 , Aline de Piano Ganen  1 , Lila Missae Oyama  1
Affiliations

Coacervate whey protein improves inflammatory milieu in mice fed with high-fat diet

Mayara Franzoi Moreno et al. Nutr Metab (Lond). .

Abstract

Background: Functional foods with bioactive properties may help in treat obesity, as they can lead to a decreased risks of inflammatory diseases. The aim of this study was to investigate the effects of chitosan coacervate whey protein on the proinflammatory processes in mice fed with high-fat diet.

Methods: Mice were divided into two groups receiving either a normolipidic or high-fat diet; the animals in each of the two diet groups were given a diet supplement of either coacervate (gavage, 36 mg protein/kg of body weight) or tap water for four weeks [groups: normolipidic diet plus water (C); normolipidic diet and coacervate (CC); high-fat diet and water (H); and high-fat diet and coacervate (HC)].

Results: The high-fat diet promoted inflammation, possibly by decreased adiponectin/sum of adipose tissues ratio and increased phosphorylation of NF-κB p50. In HC we observed a positive correlation between IL-10 and TNF-α in mesenteric adipose tissue, retroperitoneal adipose tissue and liver tissue. We also observed a positive correlation between lipopolisaccharide with IL-10 in the liver tissue.

Conclusions: High-fat diet treatment promoted metabolic alterations and inflammation, and chitosan coacervate whey protein modulated inflammatory milieu.

Keywords: Cytokines; IL-10; Mesenteric adipose tissue.

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Figures

Figure 1
Figure 1
Average body mass (g) per week in the experimental groups treated with high-fat or control diet (cycle I); and high-fat diet or control associated with gavage of water or coacervate (cycle II); (n = 9-14). *different from C (p = 0.04); †different from C, CC and H (p < 0.02).
Figure 2
Figure 2
Western blot analysis of p-pNF-kBp65, p-NF-kBp50, p-IκB, TLR4, GPx-3, SOD-1 protein in MES adipose tissue in different groups. Data are mean ± SEM (n = 4–9); *different from C (p ≤ 0.01).
Figure 3
Figure 3
Positive correlation between IL-10 and TNF-α in liver, mesenteric adipose tissue, and retroperitoneal adipose tissue. Rows represent tissue, and columns groups (n = 7-9).
Figure 4
Figure 4
Correlation between LPS and IL-10 in liver (n = 6-8).
See this image and copyright information in PMC

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