Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2014:541:151-9.
doi: 10.1016/B978-0-12-420119-4.00012-4.

One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE)

Julie L Brunelle  1 , Rachel Green  2
Affiliations

One-dimensional SDS-polyacrylamide gel electrophoresis (1D SDS-PAGE)

Julie L Brunelle et al. Methods Enzymol. 2014.

Abstract

This protocol describes a denaturing polyacrylamide gel system utilizing sodium dodecyl sulfate (SDS) to separate protein molecules based on size as first described by Laemmli (1970). SDS-PAGE can be used to monitor protein purifications, check the purity of samples, and to estimate molecular weights for unknown proteins.

Keywords: Ammonium persulfate (APS); One-dimensional SDS-Polyacrylamide Gel Electrophoresis (1D SDS-PAGE); Polyacrylamide; Polyacrylamide gel electrophoresis; SDS-PAGE gel; Stacking gel; Tetramethylethylenediamine (TEMED).

PubMed Disclaimer

Publication types

MeSH terms

LinkOut - more resources