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. 2014 Jun;16(3):449-58.
doi: 10.1007/s10544-014-9847-3.

Antibacterial performance of nanocrystallined titania confined in mesoporous silica nanotubes

Krzysztof Cendrowski  1 , Magdalena Peruzynska, Agata Markowska-Szczupak, Xuecheng Chen, Anna Wajda, Joanna Lapczuk, Mateusz Kurzawski, Ryszard J Kalenczuk, Marek Drozdzik, Ewa Mijowska
Affiliations

Antibacterial performance of nanocrystallined titania confined in mesoporous silica nanotubes

Krzysztof Cendrowski et al. Biomed Microdevices. 2014 Jun.

Abstract

In this paper, we study synthesis and characteristics of mesoporous silica nanotubes modified by titanium dioxide, as well as their antimicrobial properties and influence on mitochondrial activity of mouse fibroblast L929. Nanocrystalized titania is confined in mesopores of silica nanotubes and its light activated antibacterial response is revealed. The analysis of the antibacterial effect on Escherichia coli. (ATCC 25922) shows strong enhancement during irradiation with the artificial visible and ultraviolet light in respect to the commercial catalyst and control sample free from the nanomaterials. In darkness, the mesoporous silica/titania nanostructures exhibited antibacterial activity dependent on the stirring speed of the suspension containing nanomaterials. Obtained micrograph proved internalization of the sample into the microorganism trough the cell membrane. The analysis of the mitochondrial activity and amount of lactate dehydrogenase released from mouse fibroblast cells L929 in the presence of the sample were determined with LDH and WST1 assays, respectively. The synthesized silica/titania antibacterial agent also exhibits pronounced photoinduced inactivation of the bacterial growth under the artificial visible and UV light irritation in respect to the commercial catalyst. Additionally, mesoporous silica/titania nanotubes were characterized in details by means of high resolution transmission electron microscopy (HR-TEM), XRD and BET Isotherm.

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Figures

Fig. 1
Fig. 1
Draft and TEM images of mesoporous silica carbon nanotubes tSiO2/CNT (a, a’, a”), mesoporous silica carbon nanotubes supported with titania CNT-tSiO2-TiO2 (b, b’, b”), and mesoporous silica nanotubes with titania tSiO2/TiO2 (c, c’, c”). EDX spectrum of nanomaterials (a”’, c”’) and EDS mapping marked with red colour - carbon and green colour - titanium signal, proving the efficiency of titania modification (b”’)
Fig. 2
Fig. 2
XRD patterns of tSiO2/TiO2 with marked main peaks from anatase and silica
Fig. 3
Fig. 3
Mitochondrial activity (WST1 - blue columns) and cytotoxicity (LDH - green columns) to L929 mouse fibroblast of mesoporous silica nanotubes (tSiO2/TiO2) supported with the titania at the concentrations from 3.125 μg/mL to 100.0 μg/mL
Fig. 4
Fig. 4
Diagram of photocatalytic-antibacterial performance (of mesoporous nanotubes tSiO2/TiO2) in darkness (Dark), under artificial visible light (Vis) and ultraviolet light (UV), compared to the performance of references sample: commercial catalyst (P25) and a blank comparator (H 2 O), tested against Escherichia coli (ATCC 25922)
Fig. 5
Fig. 5
Diagram of antibacterial performance of mesoporous nanotubes tSiO2/TiO2 in darkness with different stirring speed of the solutions, tested on Escherichia coli (ATCC 25922)
Fig. 6
Fig. 6
TEM images of the Escherichia coli bacteria exposed to mesoporous nanotubes tSiO2/TiO2, without irradiation (in darkness)
Fig. 7
Fig. 7
EDX mapping of the silicon (marked with yellow colour) and titanium (marked with green colour) elements of the Escherichia coli bacteria exposed to mesoporous nanotubes tSiO2/TiO2, without irradiation (in darkness)
See this image and copyright information in PMC

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