Rapid coating of surfaces with functionalized nanoparticles for regulation of cell behavior

Abstract

A robust monolayer of nanoparticles is formed via dip-coating of cell culture plates. These surfaces provide cell type-specific modulation of growth behavior without the uptake of nanoparticles.

Keywords: cell regulation; nanomanufacturing; nanoparticle; surface modification.

Figure 1

Preparation and characterization of TTMA AuNP layer on the polystyrene-plate surface. a) Schematic representation of the strategy to generate a AuNP monolayer on the cell-culture plate. b) Angle-resolved XPS detection of the polystyrene-plate surface with the AuNP layer. Relative atomic concentrations of C, N, O and Au are listed in the table. c) AuNPs attached to the plate with or without plasma treatment under cell-culture conditions. Each bar represents the amount of gold left in one well of a 96-well plate. The error bars represent the standard deviation of three measurements.

Figure 2

HepG2 cell culture for 24 h on plates with and without the TTMA AuNP layer. a) Optical image of HepG2 cell grown on a plasma-treated plate. b) Optical image of HepG2 cell grown on a TTMA AuNP monolayer. c) Fluorescent image of Figure 2a. F-actin was stained by Oregon Green labeled phallotoxin, and the nuclei were stained by Hoechst 33342. d) Fluorescent image of Figure 2b. The staining conditions were the same as in Figure 2c. The arrow indicates filopodia of the cell. Separated fluorescent channels of Figure 2c and d can be seen in Figure S5. e) Cell-uptake test of TTMA AuNP monolayer. The cells were cultured on the TTMA AuNP monolayer in a 24-well plate for 24 h. A 24-well plate coated with TTMA AuNPs without cells cultured on the surface was used as a control.

Figure 3

Cell viability assay for 26 different AuNP coatings (morphology changes of HepG2 cell were shown in Figure S7). a) A heat map of cell viability influenced by different AuNP coatings. b) Structures of ligands on the AuNPs. ‘Blank’ represents the cell viability on plasma-treated surface without any pretreatment, and ‘Water’ represents the cell viability on the surface pretreated with water only (no AuNP added) for 3 h and without post deposition of AuNPs.