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. 2014 Feb;193(2):110-3.
doi: 10.1016/j.molbiopara.2014.03.004. Epub 2014 Mar 25.

ORC1/CDC6 and MCM7 distinct associate with chromatin through Trypanosoma cruzi life cycle

Simone Calderano  1 , Patricia Godoy  2 , Daiane Soares  1 , Osvaldo Augusto Sant'Anna  3 , Sergio Schenkman  4 , M Carolina Elias  5
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ORC1/CDC6 and MCM7 distinct associate with chromatin through Trypanosoma cruzi life cycle

Simone Calderano et al. Mol Biochem Parasitol. 2014 Feb.

Abstract

Trypanosoma cruzi alternates between replicative and non-replicative stages. We analyzed the expression of components of the pre-replication machinery TcORC1/CDC6 and TcMCM7 and their interaction with DNA in all T. cruzi stages. TcORC1/CDC6 remains in the nuclear space during all stages of the life cycle and interacts with DNA in the replicative stages; however, it does not bind to DNA in the non-replicative forms. Moreover, TcMCM7 is not present in the non-replicative stages. These data suggest that the lacking of DNA replication during the T. cruzi life cycle may be a consequence of the blocking of TcORC1/CDC6-DNA interaction and of the down regulation of the TcMCM7 expression.

Keywords: DNA replication; MCM7; ORC1/CDC6; Trypanosoma cruzi.

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