Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comment
. 2014 Apr;11(4):360-1.
doi: 10.1038/nmeth.2892.

Single-cell in situ RNA profiling by sequential hybridization

Eric Lubeck  1 , Ahmet F Coskun  1 , Timur Zhiyentayev  2 , Mubhij Ahmad  2 , Long Cai  2
Affiliations
Comment

Single-cell in situ RNA profiling by sequential hybridization

Eric Lubeck et al. Nat Methods. 2014 Apr.
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
Sequential barcoding. (a) Schematic of sequential barcoding. In each round of hybridization, 24 probes are hybridized on each transcript, imaged and then stripped by DNAse I treatment. The same probe sequences are used in different rounds of hybridization, but probes are coupled to different fluorophores. (b) Composite four-color FISH Data from 3 rounds of hybridizations on multiple yeast cells. Twelve genes are encoded by 2 rounds of hybridization, with the third hybridization using the same probes as hybridization 1. The boxed regions are magnified in the bottom right corner of each image. The matching spots are shown and barcodes are extracted. Spots without colocalization are due to nonspecific binding of probes in the cell as well as mis-hybridization. The number of each barcode can be quantified to provide the abundances of the corresponding transcripts in single cells.
See this image and copyright information in PMC

Comment on

References

    1. Lubeck E, Cai L. Nat. Methods. 2012;9:743–748. - PMC - PubMed
    1. Ke R, et al. Nat. Methods. 2013;10:857–860. - PubMed
    1. Levesque MJ, et al. Nat. Methods. 2013;10:865–867. - PMC - PubMed
    1. Levesque MJ, Raj A. Nat Meth. 2013;10:246–248. - PMC - PubMed

MeSH terms