Effects of the duper mutation on responses to light: parametric and nonparametric responses, range of entrainment, and masking

Abstract

The duper mutation in Syrian hamsters shortens the free-running period (τDD) of locomotor activity by approximately 1 h when expressed on the wild-type background and by 2 h on the tau mutant background ("super duper"). In either case, duper markedly amplifies the phase response curve (PRC) of the light pulse. This work examined whether the duper mutation alters parametric as well as nonparametric properties, intensity thresholds, and noncircadian responses to light. Furthermore, it assessed the effects of duper on the range of entrainment and circadian aftereffects. In the first study, duper mutant and wild-type (wt) hamsters showed a similar intensity threshold for light-induced phase shifts. In the second, wt, tau mutant, and super duper hamsters were exposed to LD cycles whose period (T) progressively shortened. Regardless of whether the light phase was held at 50% of T or fixed at 3 h, super duper mutants entrained to a wider range of T cycles and showed aftereffects upon release into DD. In the third study, τLL was measured in mutant and wt hamsters that were maintained for 30-day intervals in constant light of progressively greater intensities. With increasing light intensity, the circadian period shortened in duper mutants. Circadian rhythms of super duper hamsters were disrupted at light intensities considerably below those that induced arrhythmicity in wt, tau heterozygote, or duper homozygote hamsters. In the fourth study, hamsters that were wt or homozygous for duper received two 15-min light pulses: the first at CT14 to CT16 or CT17 to CT19 and the second 2 h later. As expected, wt and duper mutants showed weak and strong resetting, respectively. Light pulses in early subjective night had an additive effect in mutant but not in wt hamsters, indicating that larger phase shifts of the pacemaker take longer to complete. Finally, super duper hamsters showed slightly but not significantly more negative masking than did wt or duper mutant hamsters. These results indicate that the duper mutation affects the properties of the central circadian pacemaker. The mutant allele affects not only the PRC but also parametric responses to light.

Keywords: duper; entrainment; hamster; phase response curve; tau mutation.